Hotspot

ndhF-rpl32

SSC · 104778-105634 · 857 bp

Back to Elytrophorus

Pi 0.00703

MarkerSeek score 49.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 857
variable_sites: 6
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.0	57.9	1462 bp	1.0	79.8
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.0	57.9	1461 bp	1.0	79.8
ndhF-rpl32_p3	ACACATTCCCACAAGTTCCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1984-1985 bp	1.0	79.5
ndhF-rpl32_p4	CACATTCCCACAAGTTCCCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1983-1984 bp	1.0	79.4
ndhF-rpl32_p5	ACACATTCCCACAAGTTCCC	AATTTCACCATGGCGGCTAA	50.0	45.0	58.0	58.2	1962-1963 bp	1.0	79.2