Hotspot

psbE-petL

LSC · 62832-64132 · 1301 bp

Back to Elytrophorus

Pi 0.00538

MarkerSeek score 56.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 7
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCA	45.0	45.0	58.0	57.3	1459-1466 bp	1.0	75.0
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.0	57.3	1458-1465 bp	1.0	75.0
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1448-1455 bp	1.0	74.7
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1447-1454 bp	1.0	74.7
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	58.2	57.3	1449-1456 bp	1.0	73.2