Hotspot

petA-psbJ

LSC · 61131-62062 · 932 bp

Back to Elytrophorus

Pi 0.00859

MarkerSeek score 63.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 8
pi: 0.00859
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TGGTTCGTATTCTGGATTGGG	50.0	47.6	57.9	57.7	1068-1069 bp	1.0	70.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	1067-1068 bp	1.0	70.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TGGTTCGTATTCTGGATTGGG	50.0	47.6	58.7	57.7	1075-1076 bp	1.0	65.0
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1074-1075 bp	1.0	65.0
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TGGTTCGTATTCTGGATTGGG	45.0	47.6	57.2	57.7	1067-1068 bp	1.0	64.3