Hotspot

rbcL-psaI

LSC · 57106-57839 · 734 bp

Back to Elytrophorus

Pi 0.00274

MarkerSeek score 60.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 2
pi: 0.00274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	GCCATTGCAATTGCTGGAAA	50.0	45.0	58.0	58.2	829-841 bp	1.0	79.0
rbcL-psaI_p2	CAAGCCGGTAGATACCATCG	GCCATTGCAATTGCTGGAAA	55.0	45.0	57.9	58.2	830-842 bp	1.0	78.5
rbcL-psaI_p3	AAATTCGAGTTCAAGCCGGT	GCCATTGCAATTGCTGGAAA	45.0	45.0	58.1	58.2	841-853 bp	1.0	78.2
rbcL-psaI_p4	AAGCCGGTAGATACCATCGA	AGAAGCCATTGCAATTGCTG	50.0	45.0	58.0	57.6	833-845 bp	1.0	77.5
rbcL-psaI_p5	AGCCGGTAGATACCATCGAT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	828-840 bp	1.0	77.3