Hotspot

ndhC-trnV-UAC

LSC · 51027-51938 · 912 bp

Back to Elytrophorus

Pi 0.00768

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 7
pi: 0.00768
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCGGAGCTAAAAGTCCTGA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.4	57.9	1035-1040 bp	1.0	77.1
ndhC-trnV-UAC_p2	ACCGGAGCTAAAAGTCCTGA	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.4	57.9	1128-1133 bp	1.0	76.8
ndhC-trnV-UAC_p3	AACCGGAGCTAAAAGTCCTG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1036-1041 bp	1.0	76.1
ndhC-trnV-UAC_p4	AACCGGAGCTAAAAGTCCTG	AGCCCAATGCAGAAAAATGC	50.0	45.0	57.5	57.9	1129-1134 bp	1.0	75.9
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGAGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1045-1050 bp	1.0	75.8