Hotspot

ycf3-trnS-GGA

LSC · 45203-45773 · 571 bp

Back to Elytrophorus

Pi 0.00351

MarkerSeek score 60.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 2
pi: 0.00351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCTACAATCGAAAAGGTCT	ACGGAAAGAGAGGGATTCGA	42.9	50.0	57.3	58.2	717-718 bp	1.0	65.6
ycf3-trnS-GGA_p2	TGGCTACAATCGAAAAGGTCT	TTCCAATGCTACGCCTTGAA	42.9	45.0	57.3	57.8	667-668 bp	1.0	65.3
ycf3-trnS-GGA_p3	TGGCTACAATCGAAAAGGTCTT	ACGGAAAGAGAGGGATTCGA	40.9	50.0	58.0	58.2	717-718 bp	1.0	63.0
ycf3-trnS-GGA_p4	TTGGCTACAATCGAAAAGGTCT	ACGGAAAGAGAGGGATTCGA	40.9	50.0	58.0	58.2	718-719 bp	1.0	63.0
ycf3-trnS-GGA_p5	TGGCTACAATCGAAAAGGTCTT	TTCCAATGCTACGCCTTGAA	40.9	45.0	58.0	57.8	667-668 bp	1.0	62.7