Hotspot

trnD-GUC-psbM

LSC · 16993-17817 · 825 bp

Back to Elytrophorus

Pi 0.00972

MarkerSeek score 64.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 8
pi: 0.00972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	990-996 bp	1.0	66.6
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	1022-1028 bp	1.0	65.2
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	992-998 bp	1.0	64.7
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	991-997 bp	1.0	64.7
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	990-996 bp	1.0	64.5