Hotspot

trnG-GCC-trnfM-CAU

LSC · 12532-12896 · 365 bp

Back to Elytrophorus

Pi 0.0137
MarkerSeek score 52.70
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
365
variable_sites
5
pi
0.0137
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GAAGGTACGGGTTCGATTCC CGAGGCTCATAACCTTGAGG 55.0 55.0 58.1 57.8 443-445 bp 1.0 78.7
trnG-GCC-trnfM-CAU_p2 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 483-485 bp 1.0 78.5
trnG-GCC-trnfM-CAU_p3 GAAGGTACGGGTTCGATTCC CAGTTTGGTAGCTCACGAGG 55.0 55.0 58.1 58.3 458-460 bp 1.0 78.2
trnG-GCC-trnfM-CAU_p4 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.1 58.3 498-500 bp 1.0 78.0
trnG-GCC-trnfM-CAU_p5 GAAGGTACGGGTTCGATTCC TACAGAGCGGAGTAGAGCAG 55.0 55.0 58.1 58.3 475-477 bp 1.0 77.8