Hotspot

trnS-GCU-psbD

LSC · 8002-8984 · 983 bp

Back to Elytrophorus

Pi 0.00408

MarkerSeek score 61.40

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 4
pi: 0.00408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1128 bp	1.0	78.8
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1115 bp	1.0	78.2
trnS-GCU-psbD_p3	GTCCCAAATGATCCAAGGGG	GCCGGACCATCCTACAAAAA	55.0	50.0	58.2	58.2	1197 bp	1.0	77.3
trnS-GCU-psbD_p4	GTCCCAAATGATCCAAGGGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.2	58.2	1184 bp	1.0	76.7
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1114 bp	1.0	75.9