Hotspot

psbA

LSC · 92-1153 · 1062 bp

Pi 0.000782

MarkerSeek score 29.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 6
pi: 0.000782
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTAGAACAAGATATTGGGT	AAATGCAAGCACGATTTGGG	38.1	45.0	54.3	57.9	1462 bp	0.695652	30.1
psbA_p2	ACCCAATATCTTGCTAGAACAAG	GCGCTAGAACTTTAGCTCGT	39.1	50.0	56.3	58.1	1892 bp	0.652174	28.6
psbA_p3	ACCCAATATCTTGCTAGAACAAG	TGCGCTAGAACTTTAGCTCG	39.1	50.0	56.3	58.1	1893 bp	0.652174	28.6
psbA_p4	ACCCAATATCTTGCTAGAACAAG	AAATGCAAGCACGATTTGGG	39.1	45.0	56.3	57.9	1473 bp	0.652174	28.5
psbA_p5	ACCCAATATCTTGCTAGAACA	AAATGCAAGCACGATTTGGG	38.1	45.0	54.3	57.9	1473 bp	0.652174	28.4