Hotspot

ndhF-rpl32

SSC · 103702-104609 · 908 bp

Back to Elymus

Pi 0.00403
MarkerSeek score 59.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
908
variable_sites
20
pi
0.00403
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 976-1005 bp 1.0 43.3
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 977-1006 bp 1.0 43.3
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 978-1007 bp 1.0 43.3
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 979-1008 bp 1.0 43.3
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1003-1032 bp 1.0 43.2