Hotspot

rps8-rpl14

LSC · 77114-77254 · 141 bp

Back to Elymus

Pi 0.00454
MarkerSeek score 46.70
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
141
variable_sites
4
pi
0.00454
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TCAGCAATAGTGTCCTTGCC CGATTGCCGAGGAATTGAGA 50.0 50.0 57.9 58.1 229-237 bp 1.0 81.0
rps8-rpl14_p2 GCAATAGTGTCCTTGCCCAT CGATTGCCGAGGAATTGAGA 50.0 50.0 58.2 58.1 226-234 bp 1.0 80.1
rps8-rpl14_p3 TCAGCAATAGTGTCCTTGCC GGCAATCCAAAAGGAACTCG 50.0 50.0 57.9 57.4 260-268 bp 1.0 76.1
rps8-rpl14_p4 GCAATAGTGTCCTTGCCCAT GGCAATCCAAAAGGAACTCG 50.0 50.0 58.2 57.4 257-265 bp 1.0 75.2
rps8-rpl14_p5 TCAGCAATAGTGTCCTTGCC AGGAACTCGAGTTTTTGGCG 50.0 50.0 57.9 58.8 249-257 bp 1.0 75.1