Hotspot

psbE-petL

LSC · 62403-63572 · 1170 bp

Back to Elymus

Pi 0.00229
MarkerSeek score 50.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1170
variable_sites
15
pi
0.00229
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AGGCGTATCTTGTTCAAGCC 45.0 50.0 58.0 58.3 1370-1379 bp 1.0 79.5
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGGCGTATCTTGTTCAAGCC 50.0 50.0 57.9 58.3 1359-1368 bp 1.0 79.3
psbE-petL_p3 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.0 58.5 1324-1333 bp 1.0 77.9
psbE-petL_p4 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.0 58.5 1322-1331 bp 1.0 77.8
psbE-petL_p5 ACCAACCCGCAATGAATAGG AGGCGTATCTTGTTCAAGCC 50.0 50.0 58.2 58.3 1361-1370 bp 1.0 77.8