Hotspot

petA-psbJ

LSC · 60812-61633 · 822 bp

Back to Elymus

Pi 0.00304
MarkerSeek score 58.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
822
variable_sites
11
pi
0.00304
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GCTGGTATTGCTGTGATTGG 50.0 50.0 58.0 57.1 939-1016 bp 1.0 77.7
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC GCTGGTATTGCTGTGATTGG 50.0 50.0 57.9 57.1 922-999 bp 1.0 77.6
petA-psbJ_p3 TGTTCTTCTTCGCATCCGTT GCTGGTATTGCTGTGATTGG 45.0 50.0 57.8 57.1 915-992 bp 1.0 76.7
petA-psbJ_p4 TTGTTCTTCTTCGCATCCGT GCTGGTATTGCTGTGATTGG 45.0 50.0 57.8 57.1 916-993 bp 1.0 76.7
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT GCTGGTATTGCTGTGATTGG 50.0 50.0 58.2 57.1 936-1013 bp 1.0 76.6