Hotspot

trnF-GAA-ndhJ

LSC · 47468-48054 · 587 bp

Back to Elymus

Pi 0.00409
MarkerSeek score 51.70
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
587
variable_sites
13
pi
0.00409
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 691-697 bp 1.0 80.8
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CGCGCCTTAAACGTATCCTA 50.0 50.0 58.1 57.9 716-722 bp 1.0 80.7
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 681-687 bp 1.0 80.2
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ATGATAATCATCCGCGCCTT 50.0 45.0 58.1 57.5 728-734 bp 1.0 77.9
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA ATCCGCGCCTTAAACGTATC 50.0 50.0 58.1 58.5 719-725 bp 1.0 77.8