Hotspot

psaA-ycf3

LSC · 41084-41789 · 706 bp

Back to Elymus

Pi 0.000858
MarkerSeek score 51.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
706
variable_sites
5
pi
0.000858
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.94
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GTCTGGCCCATTCCTCAAAA TATAGCGCTTACTCCGGGAA 50.0 50.0 58.1 58.0 796-920 bp 1.0 83.7
psaA-ycf3_p2 GTCTGGCCCATTCCTCAAAA GCGCTTACTCCGGGAAATTA 50.0 50.0 58.1 58.1 792-916 bp 1.0 83.4
psaA-ycf3_p3 CTGGCCCATTCCTCAAAAGA TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 794-918 bp 1.0 82.6
psaA-ycf3_p4 TCTGGCCCATTCCTCAAAAG TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 795-919 bp 1.0 82.6
psaA-ycf3_p5 CTGGCCCATTCCTCAAAAGA GCGCTTACTCCGGGAAATTA 50.0 50.0 57.8 58.1 790-914 bp 1.0 82.3