Hotspot

trnC-GCA-rpoB

LSC · 18119-19276 · 1158 bp

Back to Elymus

Pi 0.00246
MarkerSeek score 46.60
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1158
variable_sites
16
pi
0.00246
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1225-1231 bp 1.0 80.3
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1232-1238 bp 1.0 79.2
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1240-1246 bp 1.0 78.0
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1239-1245 bp 1.0 78.0
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGGCTT 50.0 45.0 58.1 57.6 1304-1310 bp 1.0 77.6