Hotspot

trnY-GUA-trnD-GUC

LSC · 15404-15764 · 361 bp

Back to Elymus

Pi 0.00753
MarkerSeek score 58.70
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
361
variable_sites
13
pi
0.00753
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 473-491 bp 1.0 81.1
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 440-458 bp 1.0 78.9
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 439-457 bp 1.0 78.7
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 472-490 bp 1.0 77.0
trnY-GUA-trnD-GUC_p5 GATGCCCGAGTGGTTAATGG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.7 58.5 458-476 bp 1.0 76.0