Hotspot

trnT-GGU-trnE-UUC

LSC · 14723-15187 · 465 bp

Back to Elymus

Pi 0.00292
MarkerSeek score 43.50
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
465
variable_sites
8
pi
0.00292
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGTGGTAGAGTAATCTCGTGC GCTGCCTCCTTGAAAGAGAG 47.6 55.0 56.9 58.3 521-572 bp 1.0 66.5
trnT-GGU-trnE-UUC_p2 GTGGTAGAGTAATCTCGTGCT GCTGCCTCCTTGAAAGAGAG 47.6 55.0 56.9 58.3 520-571 bp 1.0 66.5
trnT-GGU-trnE-UUC_p3 AGTGGTAGAGTAATCTCGTGC TGTCCTGAACCACTAGACGA 47.6 50.0 56.9 57.7 500-551 bp 1.0 66.4
trnT-GGU-trnE-UUC_p4 GTGGTAGAGTAATCTCGTGCT TGTCCTGAACCACTAGACGA 47.6 50.0 56.9 57.7 499-550 bp 1.0 66.4
trnT-GGU-trnE-UUC_p5 GAGTAATCTCGTGCTAAGACGT GCTGCCTCCTTGAAAGAGAG 45.5 55.0 57.7 58.3 514-565 bp 1.0 64.8