Hotspot

trnS-GCU-psbD

LSC · 7565-8544 · 980 bp

Back to Elymus

Pi 0.00332
MarkerSeek score 53.60
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
980
variable_sites
23
pi
0.00332
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA GAGCAATAGGCCAGACCATC 45.0 55.0 58.0 58.1 1132-1154 bp 1.0 80.9
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1110-1132 bp 1.0 79.8
trnS-GCU-psbD_p3 ATTAGCAATCCGCCGCTTTA CCCTTCGTAACCAGTCATCC 45.0 55.0 58.0 57.7 1094-1116 bp 1.0 79.3
trnS-GCU-psbD_p4 GAGCGGAAAGAGAGGGATTC GAGCAATAGGCCAGACCATC 55.0 55.0 57.8 58.1 1183-1205 bp 1.0 79.2
trnS-GCU-psbD_p5 ATTAGCAATCCGCCGCTTTA AGGCCAGACCATCCTACAAA 45.0 50.0 58.0 58.3 1125-1147 bp 1.0 79.1