Hotspot

rpoC2

LSC · 26295-30845 · 4551 bp

Back to Elymandra

Pi 0.00309

MarkerSeek score 42.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4551
variable_sites: 21
pi: 0.00309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTGTTGGCGAAAAAGCGAAG	CCGCCTCTTCAGGAAAATGA	45.0	50.0	58.2	57.9	904-928 bp	1.0	82.3
rpoC2_p2	TAACGCCAGATCGAATCGTG	CCGCCTCTTCAGGAAAATGA	50.0	50.0	58.2	57.9	978-1002 bp	1.0	82.0
rpoC2_p3	TAACGCCAGATCGAATCGTG	TCCGCCTCTTCAGGAAAATG	50.0	50.0	58.2	57.9	979-1003 bp	1.0	82.0
rpoC2_p4	CTTTCGAGCACAACCGTTTC	TCGATTCAACAAGGTGCCAA	50.0	45.0	58.0	58.0	2359-2383 bp	1.0	81.5
rpoC2_p5	CTTTCGAGCACAACCGTTTC	TTCGATTCAACAAGGTGCCA	50.0	45.0	58.0	58.0	2360-2384 bp	1.0	81.5