Hotspot

rpl32-trnL-UAG

SSC · 107696-108256 · 561 bp

Back to Elymandra

Pi 0.0125

MarkerSeek score 59.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 561
variable_sites: 10
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	627-657 bp	1.0	81.6
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	630-660 bp	1.0	81.2
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	600-630 bp	1.0	80.9
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	593-623 bp	1.0	80.5
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	614-644 bp	1.0	80.3