Hotspot

rpl22-rps19

LSC · 81729-81815 · 87 bp

Back to Elymandra

Pi 0.00766

MarkerSeek score 62.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 1
pi: 0.00766
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTTTCTCAGCCCCTTCTTCG	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.8	58.0	229-234 bp	1.0	80.2
rpl22-rps19_p2	TTTTCTCAGCCCCTTCTTCG	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.8	58.0	230-235 bp	1.0	80.2
rpl22-rps19_p3	CGGTTGGAACTTTTCTTGCG	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.2	58.0	251-256 bp	1.0	79.5
rpl22-rps19_p4	CGGTTGGAACTTTTCTTGCG	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.2	58.0	252-257 bp	1.0	79.5
rpl22-rps19_p5	TTTTCTCAGCCCCTTCTTCG	ATGGTAGGTCGCAAATTGGG	50.0	50.0	57.8	58.2	240-245 bp	1.0	78.3