Hotspot

rps3-rpl22

LSC · 81219-81281 · 63 bp

Back to Elymandra

Pi 0.0212

MarkerSeek score 71.40

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 2
pi: 0.0212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.9	57.7	229-240 bp	1.0	78.9
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.8	57.7	195-206 bp	1.0	77.8
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	55.0	50.0	58.5	57.7	194-205 bp	1.0	75.8
rps3-rpl22_p4	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTTA	50.0	55.0	57.9	58.7	217-228 bp	1.0	75.6
rps3-rpl22_p5	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.5	57.7	193-204 bp	1.0	75.5