Hotspot

trnG-GCC-trnfM-CAU

LSC · 12838-12974 · 137 bp

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Pi 0.0292
MarkerSeek score 70.90
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
137
variable_sites
6
pi
0.0292
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AATATAGGCCGTAGCAAGCG CGAGGCTCATAACCTTGAGG 50.0 55.0 57.9 57.8 287-350 bp 1.0 79.0
trnG-GCC-trnfM-CAU_p2 AATATAGGCCGTAGCAAGCG CAGTTTTCGGCCCTACAGAG 50.0 55.0 57.9 58.3 332-395 bp 1.0 78.5
trnG-GCC-trnfM-CAU_p3 AATATAGGCCGTAGCAAGCG CAGTTTGGTAGCTCACGAGG 50.0 55.0 57.9 58.3 302-365 bp 1.0 78.5
trnG-GCC-trnfM-CAU_p4 AATATAGGCCGTAGCAAGCG CTACAGAGCGGAGTAGAGCA 50.0 55.0 57.9 58.3 320-383 bp 1.0 78.1
trnG-GCC-trnfM-CAU_p5 AATATAGGCCGTAGCAAGCG TACAGAGCGGAGTAGAGCAG 50.0 55.0 57.9 58.3 319-382 bp 1.0 78.1