Hotspot

trnS-GCU-psbD

LSC · 8320-9266 · 947 bp

Back to Elymandra

Pi 0.00495

MarkerSeek score 62.80

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 7
pi: 0.00495
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1096-1148 bp	1.0	80.0
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1095-1147 bp	1.0	79.7
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1092-1144 bp	1.0	79.4
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1079-1131 bp	1.0	78.8
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ATAAGCCGGACCATCCTACA	50.0	50.0	57.6	57.9	1082-1134 bp	1.0	77.0