Hotspot

ndhG-ndhI

SSC · 116334-116685 · 352 bp

Back to Elsholtzia

Pi 0.0592

MarkerSeek score 72.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 352
variable_sites: 93
pi: 0.0592
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAACGGTCCAAAGATGAA	ACAAACAATACGAGCTGCCA	50.0	45.0	58.1	58.1	1081-1118 bp	1.0	90.4
ndhG-ndhI_p2	CCCAACGGTCCAAAGATGAA	ATTAGAACGTTTCCGAGGCC	50.0	50.0	58.1	58.0	973-1002 bp	0.84375	84.6
ndhG-ndhI_p3	AGACCCGGTCCTAGAAAGAC	ATTAGAACGTTTCCGAGGCC	55.0	50.0	58.2	58.0	741-770 bp	0.84375	84.4
ndhG-ndhI_p4	GACCCGGTCCTAGAAAGACT	ATTAGAACGTTTCCGAGGCC	55.0	50.0	58.2	58.0	740-769 bp	0.84375	84.4
ndhG-ndhI_p5	ATGATCCCATACCAGGCTGT	ATTAGAACGTTTCCGAGGCC	50.0	50.0	58.2	58.0	1081-1085 bp	0.1875	52.2