Hotspot

rpl32-trnL-UAG

SSC · 111063-111752 · 690 bp

Back to Elsholtzia

Pi 0.0514

MarkerSeek score 80.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 690
variable_sites: 160
pi: 0.0514
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGAAAGGCTATTCGGCAG	GGTAGCAAAACCCTGGGAAA	50.0	50.0	58.0	58.0	1261-1321 bp	0.65625	77.9
rpl32-trnL-UAG_p2	AAGGAAAGGCTATTCGGCAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	791-842 bp	0.65625	76.9
rpl32-trnL-UAG_p3	AAGGAAAGGCTATTCGGCAG	AAGACCCGAGGTAGCAAAAC	50.0	50.0	58.0	57.8	1270-1330 bp	0.65625	76.5
rpl32-trnL-UAG_p4	AAGGAAAGGCTATTCGGCAG	ATGCCCCTTTCGGATGAATC	50.0	50.0	58.0	58.0	1053-1113 bp	0.59375	75.3
rpl32-trnL-UAG_p5	AAGGAAAGGCTATTCGGCAG	GCCCCTTTCGGATGAATCAT	50.0	50.0	58.0	58.0	1051-1111 bp	0.59375	75.3