Hotspot

ndhF-rpl32

SSC · 110488-110888 · 401 bp

Back to Elsholtzia

Pi 0.0494

MarkerSeek score 77.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 90
pi: 0.0494
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CACCCATATTGATATGTCTGTTGC	TGACCCAGAAGTACGTTTTT	41.7	40.0	58.0	54.8	448-479 bp	0.90625	48.3
ndhF-rpl32_p2	CACCCATATTGATATGTCTGTTGC	TTGACCCAGAAGTACGTTTT	41.7	40.0	58.0	54.8	449-480 bp	0.90625	48.3
ndhF-rpl32_p3	CACCCATATTGATATGTCTGTTGC	TTTGACCCAGAAGTACGTTT	41.7	40.0	58.0	54.8	450-481 bp	0.90625	48.3
ndhF-rpl32_p4	CACCCATATTGATATGTCTGTTGC	TTTTGACCCAGAAGTACGTT	41.7	40.0	58.0	54.8	451-482 bp	0.90625	48.3
ndhF-rpl32_p5	CACCCATATTGATATGTCTGTTGC	TTTTTGACCCAGAAGTACGT	41.7	40.0	58.0	54.8	452-483 bp	0.90625	48.3