Hotspot

trnW-CCA-trnP-UGG

LSC · 65291-65453 · 163 bp

Back to Elsholtzia

Pi 0.0631

MarkerSeek score 73.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 43
pi: 0.0631
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCGAACTGAACTAAGAGCG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.7	57.9	204-267 bp	1.0	88.3
trnW-CCA-trnP-UGG_p2	ACCGAACTGAACTAAGAGCG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.7	58.2	203-266 bp	1.0	87.8
trnW-CCA-trnP-UGG_p3	ACCGAACTGAACTAAGAGCG	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.7	57.8	196-259 bp	1.0	87.7
trnW-CCA-trnP-UGG_p4	ACCGAACTGAACTAAGAGCG	TAGCGCGTTTGTTTTGGGTA	50.0	45.0	57.7	58.4	199-262 bp	1.0	85.9
trnW-CCA-trnP-UGG_p5	CCGAACTGAACTAAGAGCGC	CTTGGTAGCGCGTTTGTTTT	55.0	45.0	59.0	57.9	203-266 bp	1.0	83.0