Hotspot

ycf4-cemA

LSC · 59458-60140 · 683 bp

Back to Elsholtzia

Pi 0.0376

MarkerSeek score 77.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 115
pi: 0.0376
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTAGCCTATTTCTTGCGCGT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.0	58.1	768-802 bp	1.0	91.2
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.9	58.1	755-789 bp	1.0	90.7
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	757-791 bp	1.0	90.4
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	760-794 bp	0.96875	87.1
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.4	58.1	764-798 bp	0.96875	86.8