Hotspot

ndhC-trnV-UAC

LSC · 49261-50195 · 935 bp

Back to Elsholtzia

Pi 0.0403

MarkerSeek score 79.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 175
pi: 0.0403
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	959-1080 bp	1.0	85.4
ndhC-trnV-UAC_p2	CGGGGCTAAAATCCCAGAAA	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.9	57.1	958-1079 bp	1.0	83.4
ndhC-trnV-UAC_p3	CTCCGGCCCTTTTCTAATCG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.4	58.7	977-1098 bp	1.0	83.2
ndhC-trnV-UAC_p4	AATCGGGGCTAAAATCCCAG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.6	58.7	962-1083 bp	0.9375	81.1
ndhC-trnV-UAC_p5	AATCGGGGCTAAAATCCCAG	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.6	57.1	961-1082 bp	0.9375	79.1