Hotspot

trnS-GCU-trnG-UCC

LSC · 8127-8797 · 671 bp

Back to Elsholtzia

Pi 0.0536

MarkerSeek score 80.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 161
pi: 0.0536
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1298-1482 bp	1.0	91.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1297-1481 bp	1.0	91.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	TAGTCGACGCCAATTCAGTG	50.0	50.0	58.0	58.0	1270-1430 bp	0.6875	79.4
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TAGTCGACGCCAATTCAGTG	50.0	50.0	58.0	58.0	1269-1429 bp	0.6875	79.4
trnS-GCU-trnG-UCC_p5	CCTCGCCTTTGAGCCTTTAT	TAGTCGACGCCAATTCAGTG	50.0	50.0	58.0	58.0	1812-1972 bp	0.6875	78.3