Hotspot

trnK-UUU-rps16

LSC · 4286-4800 · 515 bp

Back to Elsholtzia

Pi 0.0464
MarkerSeek score 75.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
515
variable_sites
101
pi
0.0464
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGGTAGAAGATTCGGGGGT GCCGTACGAGGAGAAAACTT 50.0 50.0 57.7 57.9 1506-1549 bp 0.96875 87.8
trnK-UUU-rps16_p2 AGGTAGAAGATTCGGGGGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 57.7 57.9 1505-1548 bp 0.96875 87.8
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GCCGTACGAGGAGAAAACTT 50.0 50.0 58.3 57.9 854-875 bp 0.96875 86.4
trnK-UUU-rps16_p4 AAGGTAGAAGATTCGGGGGT TCTATCCCAATGAGCCGTCT 50.0 50.0 57.7 57.9 1450-1493 bp 0.78125 79.6
trnK-UUU-rps16_p5 AGGTAGAAGATTCGGGGGTT TCTATCCCAATGAGCCGTCT 50.0 50.0 57.7 57.9 1449-1492 bp 0.78125 79.6