Hotspot

trnH-GUG-psbA

LSC · 85-412 · 328 bp

Back to Elsholtzia

Pi 0.0771

MarkerSeek score 73.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 92
pi: 0.0771
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	880-1029 bp	0.96875	89.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	894-1043 bp	0.96875	89.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	893-1042 bp	0.96875	89.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	895-1044 bp	0.96875	89.6
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AAGCTCCAACAAACGGCTAA	55.0	45.0	58.1	58.0	228-377 bp	0.75	80.8