Hotspot

ycf1

SSC · 126894-132521 · 5628 bp

Back to Eleutherococcus

Pi 0.00568

MarkerSeek score 36.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 104
pi: 0.00568
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGATTCTCTATCAAATTTACTGA	TCGTTAGAAAATTTCATAGAGACA	29.2	29.2	52.4	54.1	172 bp	1.0	43.7
ycf1_p2	CTGATTCTCTATCAAATTTACTGA	TTCGTTAGAAAATTTCATAGAGACA	29.2	28.0	52.4	54.8	173 bp	1.0	43.7
ycf1_p3	CTGATTCTCTATCAAATTTACTGAT	TCGTTAGAAAATTTCATAGAGACA	28.0	29.2	53.0	54.1	172 bp	1.0	43.7
ycf1_p4	CTGATTCTCTATCAAATTTACTGA	CGTTAGAAAATTTCATAGAGACA	29.2	30.4	52.4	52.6	171 bp	1.0	43.7
ycf1_p5	CTGATTCTCTATCAAATTTACTGA	TTTCGTTAGAAAATTTCATAGAGACA	29.2	26.9	52.4	55.5	174 bp	1.0	43.7