Hotspot

ndhF

SSC · 112895-115120 · 2226 bp

Back to Eleutherococcus

Pi 0.00411

MarkerSeek score 39.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 30
pi: 0.00411
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GATGTCCTTGCCCACACTAG	TGGTCATCTCATTTGCCCAA	55.0	45.0	58.0	57.4	2598-2659 bp	1.0	77.6
ndhF_p2	GATGTCCTTGCCCACACTAG	CGAACAGGTTGGTCATCTCA	55.0	50.0	58.0	57.5	2636-2668 bp	0.923077	74.5
ndhF_p3	GATGTCCTTGCCCACACTAG	ACGAACAGGTTGGTCATCTC	55.0	50.0	58.0	57.5	2637-2669 bp	0.923077	74.5
ndhF_p4	GATGGAATCGCCCATTACGA	CGAACAGGTTGGTCATCTCA	50.0	50.0	57.8	57.5	2802-2834 bp	0.923077	73.4
ndhF_p5	GATGGAATCGCCCATTACGA	ACGAACAGGTTGGTCATCTC	50.0	50.0	57.8	57.5	2803-2835 bp	0.923077	73.3