Hotspot

ycf1-ndhF

SSC · 112860-112894 · 35 bp

Back to Eleutherococcus

Pi 0.114

MarkerSeek score 64.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 35
variable_sites: 11
pi: 0.114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GATGTCCTTGCCCACACTAG	GTTGGTCGTGTAATCGTGGT	55.0	50.0	58.0	58.2	434-495 bp	1.0	85.1
ycf1-ndhF_p2	GATGTCCTTGCCCACACTAG	TGGTTGGTCGTGTAATCGTG	55.0	50.0	58.0	58.2	436-497 bp	1.0	85.1
ycf1-ndhF_p3	ACGAAGGGGACTAAACAGGA	GTTGGTCGTGTAATCGTGGT	50.0	50.0	58.0	58.2	913-974 bp	1.0	83.2
ycf1-ndhF_p4	ACGAAGGGGACTAAACAGGA	TGGTTGGTCGTGTAATCGTG	50.0	50.0	58.0	58.2	915-976 bp	1.0	83.2
ycf1-ndhF_p5	ACAGGAACAAGAGGGATCCA	GTTGGTCGTGTAATCGTGGT	50.0	50.0	58.0	58.2	899-960 bp	1.0	83.1