Hotspot

trnP (UGG)-psaJ

LSC · 69368-69749 · 382 bp

Back to Eleutherococcus

Pi 0.00718
MarkerSeek score 30.80
Primer available yes
Label trnP (UGG)-psaJ

Feature Metadata

label_name
trnP (UGG)-psaJ
feature_type
igs
region
LSC
length_bp
382
variable_sites
7
pi
0.00718
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP (UGG)-psaJ_p1 ACCCGTGACATTTTGTACCC GAGAGATATGTTTTTAGATCTCGCA 50.0 36.0 58.1 56.8 463-480 bp 1.0 41.2
trnP (UGG)-psaJ_p2 CCCGTGACATTTTGTACCCA GAGAGATATGTTTTTAGATCTCGCA 50.0 36.0 58.1 56.8 462-479 bp 1.0 41.2
trnP (UGG)-psaJ_p3 ACAGGATTTGAACCCGTGAC GAGAGATATGTTTTTAGATCTCGCA 50.0 36.0 58.1 56.8 474-491 bp 1.0 41.2
trnP (UGG)-psaJ_p4 CAGGATTTGAACCCGTGACA GAGAGATATGTTTTTAGATCTCGCA 50.0 36.0 58.1 56.8 473-490 bp 1.0 41.2
trnP (UGG)-psaJ_p5 TGACAGGATTTGAACCCGTG GAGAGATATGTTTTTAGATCTCGCA 50.0 36.0 58.1 56.8 476-493 bp 1.0 41.2