Hotspot

atpB-rbcL

LSC · 57220-58023 · 804 bp

Back to Eleutherococcus

Pi 0.00282

MarkerSeek score 52.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 6
pi: 0.00282
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGTGATATTCCCCAGGTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	914-939 bp	1.0	74.0
atpB-rbcL_p2	TGGGTGATATTCCCCAGGTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	913-938 bp	1.0	73.7
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	937-962 bp	1.0	73.6
atpB-rbcL_p4	GGAAACCCCAGAACCTGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	879-904 bp	1.0	73.4
atpB-rbcL_p5	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	936-961 bp	1.0	73.3