Hotspot

ndhC-trnV (UAC)

LSC · 53172-54184 · 1013 bp

Back to Eleutherococcus

Pi 0.00675

MarkerSeek score 54.30

Primer available yes

Label ndhC-trnV (UAC)

Feature Metadata

label_name: ndhC-trnV (UAC)
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 21
pi: 0.00675
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV (UAC)_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1121-1174 bp	1.0	80.9
ndhC-trnV (UAC)_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1133-1186 bp	1.0	79.3
ndhC-trnV (UAC)_p3	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCACAAACAATG	50.0	45.0	57.9	57.8	1227 bp	0.076923	40.6
ndhC-trnV (UAC)_p4	CCTTTGCTAATCGGGGCTAA	AGCCCAATGCACAAACAATG	50.0	45.0	57.7	57.8	1239 bp	0.076923	39.0
ndhC-trnV (UAC)_p5	GGGGCTAAAACTCCGGAAAT	GAGCCCAATGCACAAACAAT	50.0	45.0	57.9	57.5	1228 bp	0.076923	38.3