Hotspot

trnK (UUU)-rps16

LSC · 4467-5336 · 870 bp

Back to Eleutherococcus

Pi 0.00966
MarkerSeek score 50.80
Primer available yes
Label trnK (UUU)-rps16

Feature Metadata

label_name
trnK (UUU)-rps16
feature_type
igs
region
LSC
length_bp
870
variable_sites
29
pi
0.00966
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK (UUU)-rps16_p1 CGAATCCTTCGCTTCATCCA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.1 58.3 1024-1080 bp 1.0 79.7
trnK (UUU)-rps16_p2 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1037-1093 bp 1.0 79.5
trnK (UUU)-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 976-1032 bp 1.0 79.0
trnK (UUU)-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 975-1031 bp 1.0 79.0
trnK (UUU)-rps16_p5 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1061-1117 bp 1.0 78.3