Hotspot

petB

LSC · 71433-72857 · 1425 bp

Back to Eleusine

Pi 0.000702

MarkerSeek score 59.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1425
variable_sites: 1
pi: 0.000702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1525 bp	1.0	67.1
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1525 bp	1.0	66.7
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1531 bp	1.0	66.3
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1530 bp	1.0	64.9
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1531 bp	1.0	64.9