Hotspot

atpF

LSC · 33478-34865 · 1388 bp

Back to Eleusine

Pi 0.00072

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1388
variable_sites: 1
pi: 0.00072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1538 bp	1.0	78.5
atpF_p2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	45.0	45.0	58.1	57.9	1539 bp	1.0	78.5
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	45.0	45.0	58.1	57.9	1540 bp	1.0	78.5
atpF_p4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	45.0	45.0	58.1	57.9	1541 bp	1.0	78.5
atpF_p5	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTAGTG	45.0	50.0	58.1	57.9	1575 bp	1.0	78.3