Hotspot

ndhF-rpl32

SSC · 103768-104702 · 935 bp

Back to Eleusine

Pi 0

MarkerSeek score 59.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 935
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAAGAAAAAGCCCCAACCCC	TGACATCGAAGTACGTTTTT	50.0	35.0	58.0	53.2	1052-1053 bp	1.0	42.0
ndhF-rpl32_p2	TAAGAAAAAGCCCCAACCCC	TTGACATCGAAGTACGTTTT	50.0	35.0	58.0	53.2	1053-1054 bp	1.0	42.0
ndhF-rpl32_p3	TAAGAAAAAGCCCCAACCCC	TTTGACATCGAAGTACGTTT	50.0	35.0	58.0	53.2	1054-1055 bp	1.0	42.0
ndhF-rpl32_p4	TAAGAAAAAGCCCCAACCCC	TTTTGACATCGAAGTACGTT	50.0	35.0	58.0	53.2	1055-1056 bp	1.0	42.0
ndhF-rpl32_p5	TAAGAAAAAGCCCCAACCCC	TTTTTGACATCGAAGTACGT	50.0	35.0	58.0	53.2	1056-1057 bp	1.0	42.0