Hotspot

rbcL-psaI

LSC · 56762-57646 · 885 bp

Back to Eleusine

Pi 0.00339

MarkerSeek score 60.70

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 3
pi: 0.00339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	979 bp	1.0	80.0
rbcL-psaI_p2	ACATTTGACTTCAAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	991 bp	1.0	79.9
rbcL-psaI_p3	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	980 bp	1.0	79.5
rbcL-psaI_p4	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	985 bp	1.0	79.3
rbcL-psaI_p5	ACATTTGACTTCAAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	997 bp	1.0	79.3