Hotspot

trnS-GGA-rps4

LSC · 45288-45556 · 269 bp

Back to Eleusine

Pi 0

MarkerSeek score 19.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GGTTGTGGCTGTACCTGTAC	TCATTCGATCCGGGCAAATT	55.0	45.0	58.2	57.9	580 bp	1.0	78.2
trnS-GGA-rps4_p2	GGTTGTGGCTGTACCTGTAC	TTCATTCGATCCGGGCAAAT	55.0	45.0	58.2	57.9	581 bp	1.0	78.2
trnS-GGA-rps4_p3	GGTTGTGGCTGTACCTGTAC	TCATGTCCCGTTATCGAGGA	55.0	50.0	58.2	57.9	1041 bp	1.0	78.0
trnS-GGA-rps4_p4	GGTTGTGGCTGTACCTGTAC	CATTCGATCCGGGCAAATTG	55.0	50.0	58.2	57.9	579 bp	1.0	77.6
trnS-GGA-rps4_p5	GGTTGTGGCTGTACCTGTAC	CATGTCCCGTTATCGAGGAC	55.0	55.0	58.2	57.9	1040 bp	1.0	77.4