Hotspot

trnT-GGU-trnE-UUC

LSC · 14866-15413 · 548 bp

Back to Eleusine

Pi 0.00183

MarkerSeek score 58.90

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 1
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	668-669 bp	1.0	67.3
trnT-GGU-trnE-UUC_p2	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	647-648 bp	1.0	67.2
trnT-GGU-trnE-UUC_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	631-632 bp	1.0	67.2
trnT-GGU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	610-611 bp	1.0	67.0
trnT-GGU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	636-637 bp	1.0	66.4