Hotspot

trnG-GCC-trnfM-CAU

LSC · 12536-12920 · 385 bp

Back to Eleusine

Pi 0

MarkerSeek score 41.40

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTTCGATTCCCGTCGCTC	AGTTTTTGGCCCTACAGAGC	61.1	50.0	58.3	58.1	496-497 bp	1.0	61.0
trnG-GCC-trnfM-CAU_p2	GTTCGATTCCCGTCGCTC	CGAGGCTCATAACCTTGAGG	61.1	55.0	58.3	57.8	452-453 bp	1.0	59.8
trnG-GCC-trnfM-CAU_p3	GTTCGATTCCCGTCGCTC	CAGTTTGGTAGCTCACGAGG	61.1	55.0	58.3	58.3	467-468 bp	1.0	59.4
trnG-GCC-trnfM-CAU_p4	GTTCGATTCCCGTCGCTC	CCAGTTTTTGGCCCTACAGA	61.1	50.0	58.3	57.7	498-499 bp	1.0	59.4
trnG-GCC-trnfM-CAU_p5	GTTCGATTCCCGTCGCTC	TACAGAGCGGAGTAGAGCAG	61.1	55.0	58.3	58.3	484-485 bp	1.0	59.0