Hotspot

matK-trnK-UUU

LSC · 3209-3880 · 672 bp

Back to Eleusine

Pi 0.00149
MarkerSeek score 59.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
672
variable_sites
1
pi
0.00149
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CATAATCGTGGGCAAATGCA AGGAGCGTTCGGTGTAATTC 45.0 50.0 57.1 58.0 880 bp 1.0 73.1
matK-trnK-UUU_p2 ACATAATCGTGGGCAAATGC AGGAGCGTTCGGTGTAATTC 45.0 50.0 57.1 58.0 881 bp 1.0 73.1
matK-trnK-UUU_p3 CATAATCGTGGGCAAATGCA CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 881 bp 1.0 70.9
matK-trnK-UUU_p4 ACATAATCGTGGGCAAATGC CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 882 bp 1.0 70.9
matK-trnK-UUU_p5 CATAATCGTGGGCAAATGCA GAGCGTTCGGTGTAATTCCA 45.0 50.0 57.1 58.3 878 bp 1.0 70.9